Determining Cell Number During Cell Culture using the Scepter Cell Counter

Overview

The Scepter Cell Counter is a handheld automated device designed for counting cells, monitoring cell diameter and volume, and assessing the health of cellular populations. It provides a user-friendly and cost-effective alternative to traditional counting methods.

Key Study Components

Area of Science

• Cell Biology
• Cell Culture Techniques
• Automated Cell Counting

Background

• Cell counting is essential for ensuring experimental reproducibility and accuracy.
• Traditional methods like hemocytometry are labor-intensive and prone to user bias.
• Automated devices can improve accuracy but are often expensive.
• The Scepter Cell Counter offers a low-cost, handheld solution for automated counting.

Purpose of Study

• To demonstrate the use of the Scepter Cell Counter for accurate cell counting.
• To highlight the advantages of using an automated device over manual methods.
• To provide a step-by-step guide on operating the Scepter Cell Counter.

Methods Used

• Cells are diluted in phosphate buffered saline before counting.
• A disposable sensor is used to aspirate the cell suspension.
• The device counts cells and displays statistics on a graphical interface.
• Data can be uploaded to a computer for further analysis.

Main Results

• The Scepter Cell Counter provides accurate cell counts and population statistics.
• It displays results as histograms for easy interpretation.
• Users can apply gates to refine data based on size and volume.
• Up to 72 histograms can be stored for later analysis.

Conclusions

• The Scepter Cell Counter is an effective tool for cell counting in research.
• It reduces the time and effort required compared to manual counting methods.
• The device enhances accuracy and reproducibility in cell culture experiments.

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