This study demonstrates a method for targeted delivery of therapeutic nanoparticles to glioblastoma multiforme in a mouse model. The nanoparticles, composed of an iron oxide core and conjugated with therapeutic oligonucleotides, facilitate both treatment and imaging of the tumor.
Begin with an anesthetized nude mouse model bearing a glioblastoma multiforme, a high-grade malignant brain tumor.
Draw the appropriate volume of the nanoparticle solution into a syringe, avoiding air bubbles.
The nanoparticles consist of an iron oxide core covered with dextran, which is conjugated to a therapeutic oligonucleotide to inhibit tumor growth, and to a fluorescent dye to enable imaging.
Disinfect the tail with isopropanol and rotate it to expose the tail vein.
Gently bend the needle, insert it into the vein, and pull back the plunger slightly to create a vacuum.
Advance the needle until blood enters the syringe, confirming vein access.
Slowly inject the nanoparticle solution, then retract the needle.
Once in circulation, the nanoparticles, due to their small size, cross the blood-brain barrier and accumulate in the glioblastoma within the brain.
This technique enables targeted delivery of the therapeutic agent to the tumor site and facilitates non-invasive imaging.
Weigh the mouse and calculate the volume of magnetic nanoparticle, anti-microRNA-10b to administer for a dosage of 20 milligrams iron per kilogram in glioblastoma multiforme. Without introducing bubbles, prepare a 28-gauge syringe with the dose of magnetic nanoparticle anti-microRNA-10b.
Sterilize the mouse tail with a 70 percent isopropanol wipe. After drying, rotate the tail to position the lateral caudal veins at the top of the tail. Then insert the needle into the tail and pull back the plunger to establish a vacuum, continue inserting the needle until the blood enters the syringe.
Slowly inject magnetic nanoparticle solution and retract the needle after complete delivery. Apply pressure to the injection site with gauze until the bleeding stops.
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