Overview
This article describes a method for converting an HP DeskJet 500 printer into a bioprinter capable of processing living cells. The technique creates transient pores in cell membranes, allowing for the incorporation of small molecules, such as fluorescent G-actin, into the printed cells.
Key Study Components
Area of Science
- Bioprinting
- Cell biology
- Tissue engineering
Background
- Traditional methods for introducing molecules into cells include transfection and microinjection.
- These methods can be complex and may affect cell viability.
- Inkjet printing offers a simpler alternative with high cell viability.
- This study explores the use of a modified printer for bioprinting applications.
Purpose of Study
- To develop a bioprinting method using an HP DeskJet 500 printer.
- To demonstrate the incorporation of fluorescent G-actin into living cells.
- To provide insights into cytoskeleton dynamics during cell migration.
Methods Used
- Modification of an HP DeskJet 500 printer for bioprinting.
- Preparation of bio ink using fluorescently tagged actin monomers and cell suspension.
- Printing bio ink onto microscope slides.
- Fluorescent microscopy to visualize internalization of actin monomers by printed cells.
Main Results
- Successful printing of fibroblast cells onto glass slides.
- Demonstration of incorporated fluorescent actin monomers within cells.
- High cell viability compared to traditional methods.
- Visualization of cytoskeleton changes in response to applied forces.
Conclusions
- The modified printer effectively creates transient pores in cell membranes.
- This bioprinting technique is user-friendly and maintains high cell viability.
- It has potential applications in genetics, cell biophysics, and tissue engineering.
What is bioprinting?
Bioprinting is a technique that uses printers to deposit living cells and biomaterials in a controlled manner.
How does the bioprinter work?
The bioprinter modifies a standard printer to create transient pores in cell membranes, allowing for the incorporation of bio ink.
What are the advantages of this method?
This method is easier to use and yields higher cell viability compared to traditional transfection techniques.
What applications does this technique have?
It can be applied in genetics, cell biophysics, and tissue engineering.
What is fluorescent G-actin?
Fluorescent G-actin is a tagged version of actin monomers that can be visualized under a fluorescence microscope.
How are the cells prepared for printing?
Cells are harvested, centrifuged, and resuspended in a solution of fluorescent G-actin to create the bio ink.
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