Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

作者： Miquella G. Chavez1,2, Jimmy Hu1, Kerstin Seidel1, Chunying Li1,3, Andrew Jheon1, Adrien Naveau4,5,6, Orapin Horst1,7, Ophir D. Klein1,8 1Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology,University of California, San Francisco, 2Department of Bioengineering and Therapeutic Sciences,University of California, San Francisco, 3Department of Pathology and Research Center,Zhongshan Hospital of Dalian University, 4Université Paris Descartes, Sorbonne Paris Cite, UMR S872, 5Centre de Recherche des Cordeliers,Université Pierre et Marie Curie, UMR S872, 6INSERM U872, 7Division of Endodontics, Department of Preventive and Restorative Dental Sciences,University of California, San Francisco, 8Department of Pediatrics and Institute for Human Genetics,University of California, San Francisco

简介：

Overview

This article presents a reliable method for isolating dental epithelial stem cells (DESCs) from the continuously growing mouse incisor. The procedure involves dissection and microdissection techniques to obtain and culture these cells effectively.

Key Study Components

Area of Science

• Neuroscience
• Stem Cell Biology
• Dental Research

Background

• The mouse incisor serves as a model for studying dental tissue renewal.
• Dental epithelial stem cells (DESCs) are crucial for understanding tooth development.
• Reliable methods for cell isolation are essential for in vitro studies.
• This research contributes to the field of regenerative medicine.

Purpose of Study

• To establish a consistent protocol for obtaining DESC from mouse incisors.
• To enhance the understanding of dental tissue regeneration.
• To provide a foundation for future research on dental stem cells.

Methods Used

• Dissection of the lower hemi mandible from adult mice.
• Removal of bone covering the cervical loop region.
• Isolation and separation of the cervical loop region from the epithelium.
• Microdissection of the cervical loop followed by cell dissociation and culture.

Main Results

• A reliable method for isolating DESC was successfully established.
• The procedure allows for consistent cell culture from the mouse incisor.
• Video demonstration aids in understanding the isolation process.
• Results contribute to the field of dental tissue engineering.

Conclusions

• The study provides a robust protocol for DESC isolation.
• Findings may facilitate further research in dental regeneration.
• Future studies can build upon this method for various applications.

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