Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

作者： Jack C. Bridge1, Jonathan W. Aylott2, Christopher E. Brightling5, Amir M. Ghaemmaghami3, Alan J. Knox4, Mark P. Lewis6, Felicity R.A.J. Rose1, Gavin E. Morris1 1Division of Drug Delivery and Tissue Engineering,University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy,University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences,University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences,University of Nottingham, 5NIHR Respiratory Biomedical Research Unit,University of Leicester, 6School of Sport, Exercise, and Health Sciences,Loughborough University

简介：

Overview

This study presents a method for creating three-dimensional multi-cell-type constructs using electrospinning techniques. The developed scaffolds aim to replicate the airway bronchiole wall, facilitating the co-culture of differentiated human cells.

Key Study Components

Area of Science

• Biomaterials
• Tissue Engineering
• Cell Culture

Background

• Advancements in biomaterial technologies have enabled the development of complex tissue models.
• Existing methods often rely on 2D cultures, which do not accurately represent in vivo environments.
• 3D scaffolds can enhance cellular interactions and mimic the extracellular matrix.
• This study focuses on airway structural cells and their interactions in a controlled environment.

Purpose of Study

• To develop a 3D in vitro model of the airway bronchiole wall.
• To facilitate the co-culture of multiple cell types for better disease modeling.
• To reduce reliance on animal models in research.

Methods Used

• Electrospinning of microfiber and nanofiber scaffolds.
• Co-culturing of differentiated adult human cells.
• Use of a perfused bioreactor system for enhanced nutrient delivery.
• Measurement of cellular interactions through mediator release and immunostaining.

Main Results

• The electrospun scaffolds successfully supported the growth of multiple cell types.
• 3D constructs demonstrated improved cellular interactions compared to traditional 2D cultures.
• Potential applications include studying diseases like asthma and COPD.
• The method provides a stable platform for in vitro experimentation.

Conclusions

• This electrospinning technique offers a promising approach for creating physiologically relevant tissue models.
• It can significantly advance research in respiratory diseases and beyond.
• Future studies may explore additional applications in other organ systems.

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