Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

作者： Christina Wayman*1,2, Denise A. Duricki*1,2, Lisa A. Roy3, Barbara Haenzi1, Shi-Yen Tsai4, Gwendolyn Kartje4,5,6, John S. Beech7, Diana Cash2, Lawrence Moon1 1Wolfson Centre for Age-Related Diseases,King's College London, University of London, 2Department of Neuroimaging, James Black Centre, Institute of Psychiatry,King's College London, University of London, 3Institute of Neuroscience and Psychology, Wellcome Surgical Institute, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow, 4Research Service,Edward Hines Jr. VA Hospital, 5Neurology Service,Edward Hines Jr. VA Hospital, 6Department of Molecular Pharmacology and Therapeutics, Neuroscience Research Institute,Loyola University Chicago, 7Department of Oncology, The Gray Institute for Radiation, Oncology and Biology,University of Oxford

简介：

Overview

This article presents a protocol for performing permanent distal middle cerebral artery occlusion in elderly female rats, aimed at generating large cortical infarcts and sustained behavioral deficits. The lesion size is confirmed using structural MRI at 24 hours and 8 weeks post-stroke.

Key Study Components

Area of Science

• Neuroscience
• Stroke Models
• Animal Surgery

Background

• Permanent MCA occlusion is a common model for studying stroke.
• Elderly rats are used to mimic age-related stroke effects.
• Behavioral deficits are assessed post-surgery.
• Structural MRI is utilized for lesion confirmation.

Purpose of Study

• To establish a reliable model for studying stroke in elderly rats.
• To evaluate the effects of MCA occlusion on brain function.
• To confirm lesion size and recovery using MRI.

Methods Used

• Preparation of the rat under anesthesia and analgesia.
• Exposure and ligation of carotid arteries.
• Craniotomy and coagulation of the MCA.
• Use of MRI to assess infarct size post-surgery.

Main Results

• Successful occlusion of the MCA was achieved.
• Large cortical infarcts were generated in the rats.
• Behavioral deficits were sustained over the study period.
• Lesion size was confirmed via MRI at specified time points.

Conclusions

• The protocol provides a reproducible model for stroke research.
• It allows for the assessment of long-term effects of MCA occlusion.
• Future studies can utilize this model to explore therapeutic interventions.

Frequently Asked Questions