A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

作者： Clovis S. Palmer1,2,3, Joshua J. Anzinger4, Tiffany R. Butterfield4, Joseph M. McCune5, Suzanne M. Crowe1,2,6 1Centre for Biomedical Research,Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases,Monash University, 3Department of Microbiology and Immunology,University of Melbourne, 4Department of Microbiology,The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine,University of California, San Francisco, 6Department of Medicine,Monash University

简介：

Overview

This article presents a flow cytometry protocol to measure glucose transporter expression and glucose uptake in monocytes, crucial components of the innate immune system. The method allows for the analysis of monocyte subpopulations in fresh whole blood, providing insights into immune cell metabolism.

Key Study Components

Area of Science

• Immunology
• Cell Metabolism
• Flow Cytometry

Background

• Monocytes play a vital role in the human immune response.
• Glycolytic metabolism is essential for activated monocytes.
• Understanding glucose uptake can inform disease outcomes.
• Flow cytometry allows for detailed analysis of immune cells.

Purpose of Study

• To measure glucose uptake in monocyte subpopulations.
• To investigate the role of immune cell metabolism in diseases.
• To provide a rapid and efficient method for analysis.

Methods Used

• Collection of blood samples in citrate ACD-B anticoagulant tubes.
• Use of a lysing solution to treat cells on ice.
• Centrifugation and washing of cells for analysis.
• Flow cytometry to measure glucose transporter expression.

Main Results

• Successful measurement of glucose uptake in monocytes.
• Identification of differences in glucose transporter expression.
• Insights into metabolic pathways in immune responses.
• Demonstration of the method's efficiency and ease of use.

Conclusions

• The method provides valuable data on monocyte metabolism.
• Understanding glucose uptake can aid in disease research.
• This protocol can be applied to various immunological studies.

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