Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

作者： Chi Ching Goh1,2, Jackson LiangYao Li1, David Becker3, Wolfgang Weninger4,5,6, Veronique Angeli2,7, Lai Guan Ng1,2,5,8 1Singapore Immunology Network (SIgN),Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology,University of Sydney, 6Department of Dermatology,Royal Prince Alfred Hospital, 7LSI Immunology Programme,National University of Singapore, 8School of Biological Sciences,Nanyang Technological University

简介：

Overview

This protocol describes the induction of an ischemia-reperfusion (IR) model on mouse ear skin using magnet clamping. The study utilizes intravital multiphoton imaging to investigate in vivo inflammatory responses post-reperfusion.

Key Study Components

Area of Science

• Immunology
• Dermatology

Background

• Understanding immune cell dynamics is crucial for addressing ischemic reperfusion injuries.
• The technique provides a robust imaging model for studying cellular activities in inflamed or resting skin.
• This method can also be applied to other conditions such as hypersensitivity reactions and infections.

Purpose of Study

• To understand how immune cells respond to ischemia-reperfusion.
• To utilize intravital imaging for real-time observation of immune responses.
• To extend knowledge in the fields of Immunology and Dermatology.

Methods Used

• Induction of ischemia-reperfusion model on mouse ear skin.
• Use of magnet clamping for model induction.
• Intravital multiphoton imaging to observe immune cell behavior.
• Maintaining mouse body temperature at 37 degrees Celsius during preparation.

Main Results

• Insights into the dynamic behavior of immune cells post-reperfusion.
• Demonstration of the imaging model's effectiveness in studying skin inflammation.
• Potential applications to other inflammatory conditions.

Conclusions

• The developed technique enhances understanding of immune responses to skin IR injury.
• It provides a valuable tool for future research in related fields.
• Further studies could explore its application in various inflammatory diseases.

Frequently Asked Questions