Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

作者： Rong Li*1, Jinxin Miao*1,2, Zhiqiang Fan1, SeokHwan Song3, Il-keun Kong3,4, Yaohe Wang2,5, Zhongde Wang1 1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, 4Institute of Agriculture and Life Science,Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London

简介：

Overview

This article presents a detailed protocol for producing genetically engineered golden Syrian hamsters using the CRISPR/Cas9 system via pronuclear injection. The method addresses the unique challenges posed by hamster embryos, enabling advancements in animal model development.

Key Study Components

Area of Science

• Genetic engineering
• Animal models
• CRISPR technology

Background

• Genetically engineered models are crucial for disease research.
• Hamster embryos are particularly sensitive to environmental conditions.
• The CRISPR/Cas9 system allows precise gene editing.
• This protocol enhances the feasibility of creating knockout hamsters.

Purpose of Study

• To develop a reliable PN injection protocol for hamsters.
• To facilitate the production of genetically modified hamster models.
• To contribute to human disease modeling through advanced animal models.

Methods Used

• Preparation of zygote handling dishes.
• Use of HECM-9 medium for PN injection.
• Detailed pronuclear injection procedures.
• Monitoring embryo development post-injection.

Main Results

• Successful production of gene knockout hamsters.
• Establishment of a robust protocol for future studies.
• Demonstration of the feasibility of genetic engineering in hamsters.
• Potential for significant contributions to biomedical research.

Conclusions

• The developed protocol is a significant advancement in genetic engineering.
• It opens new avenues for research using hamster models.
• Future studies can leverage this technique for various applications.

Frequently Asked Questions