Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

作者： Tanner J. McArdle1, Brenda M. Ogle1,2,3,4,5, Felicite K. Noubissi6 1Department of Biomedical Engineering,University of Minnesota - Twin Cities, 2Stem Cell Institute,University of Minnesota - Twin Cities, 3Masonic Cancer Center,University of Minnesota - Twin Cities, 4Lillehei Heart Institute,University of Minnesota - Twin Cities, 5Institute for Engineering in Medicine,University of Minnesota - Twin Cities, 6Department of Biology,Jackson State University

简介：

Overview

This protocol describes an inverted vertical invasion assay that quantifies the migration and invasion capabilities of rare and sensitive cells in a three-dimensional setting while preserving the cell microenvironment. This method is particularly relevant in cancer research.

Key Study Components

Area of Science

• Cell migration
• Cancer research
• Invasion assays

Background

• The assay allows for the analysis of cells in their original micro-environment.
• It is designed for rare and sensitive cells.
• Understanding cell invasion is crucial for cancer therapy development.
• Visual demonstration of the technique is essential for proper execution.

Purpose of Study

• To quantify the migratory and invasive capacities of cells.
• To explore the invasion capability of sensitive cells.
• To screen pharmacological agents that inhibit cancer cell invasion and metastasis.

Methods Used

• Inverted vertical invasion assay
• Three-dimensional cell culture
• Analysis of cells in their micro-environment
• Assessment of temperature and pH effects on collagen polymerization

Main Results

• Demonstrated the ability to analyze cell behavior in a 3D setting.
• Highlighted the importance of maintaining the cell microenvironment.
• Provided insights into the invasive capabilities of cancer cells.
• Showed potential for screening therapeutic agents.

Conclusions

• The assay is a valuable tool for cancer research.
• It preserves the microenvironment of sensitive cells.
• Can aid in the development of therapies targeting cancer invasion and metastasis.

Frequently Asked Questions