A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

作者： Zhenan Liu1, Jie Wen1,2, Xue Leng1, Qian Zhou1, Changkuo Zhou3, Huaqiang Zhao1, Xunwei Wu1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

简介：

Overview

This article presents a simplified protocol for isolating primary human keratinocytes from adult skin tissues. The method utilizes the ROCK Inhibitor Y-27632 to facilitate the separation of epidermal cells from dermal cells.

Key Study Components

Area of Science

• Cell Biology
• Dermatology
• Regenerative Medicine

Background

• Primary human keratinocytes are essential for various research applications.
• Conventional isolation methods can be complex and time-consuming.
• The use of ROCK Inhibitor Y-27632 offers a more efficient approach.
• Isolated keratinocytes can be used in both laboratory and clinical settings.

Purpose of Study

• To develop a more efficient method for isolating epidermal cells.
• To enhance the yield of high-potential epidermal cells.
• To facilitate research and clinical applications involving skin cells.

Methods Used

• Wash skin tissue with PBS before weighing.
• Weigh the skin tissue using an electronic scale.
• Rinse the tissue with 70% ethanol for 30 seconds.
• Incubate the tissue in PBS with antibiotics twice for five minutes each.

Main Results

• The protocol successfully isolates primary keratinocytes.
• High yields of viable epidermal cells were obtained.
• The method is reproducible and efficient.
• Cells isolated can be used for various applications.

Conclusions

• This method simplifies the isolation of primary keratinocytes.
• It provides a reliable approach for researchers in the field.
• The use of ROCK Inhibitor enhances cell yield and viability.

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