Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Overview

This article presents a tandem mass spectrometry-based protocol for quantifying commonly used antibiotics in intensive care units, including cefepime, meropenem, ciprofloxacin, moxifloxacin, linezolid, and piperacillin. The method is optimized for therapeutic drug monitoring to adjust dosages based on pathogen susceptibility.

Key Study Components

Area of Science

• Pharmacology
• Clinical Laboratory Science
• Mass Spectrometry

Background

• Antibiotic therapeutic drug monitoring is crucial for effective treatment.
• National and local antibiotic stewardship programs emphasize the need for precise monitoring.
• The protocol addresses the demand for accurate quantification of antibiotics in clinical settings.
• Commonly used antibiotics in intensive care units are targeted for this method.

Purpose of Study

• To provide a reliable protocol for quantifying antibiotics in circulation.
• To facilitate dosage adjustments based on the susceptibility of pathogens.
• To support daily therapeutic drug monitoring in clinical laboratories.

Methods Used

• Preparation of serum calibrators and quality controls using spike solutions.
• Use of HPLC and mass spectrometry for analysis.
• Detailed steps for sample preparation and analysis execution.
• Calibration and quality control checks to ensure accuracy.

Main Results

• The method allows for a broad calibration range suitable for pharmacokinetic calculations.
• Turnaround time for analysis is approximately 30 minutes.
• Quality control measures ensure reliable results.
• Calibration curves demonstrate linearity and accuracy within specified limits.

Conclusions

• The presented protocol is effective for daily monitoring of antibiotic levels.
• It supports clinical decision-making in antibiotic therapy.
• Future applications may enhance patient outcomes in intensive care settings.

Frequently Asked Questions