Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Overview

This article presents a protocol for a novel chemiresistive micro pH sensor that is Nafion-coated and polyaniline-functionalized. The sensor is designed to detect real-time pH changes during Lactococcus lactis fermentation, offering a compact and efficient alternative to conventional pH measurement techniques.

Key Study Components

Area of Science

• Bioprocess technology
• Microreactor systems
• Electrochemical sensing

Background

• Conventional pH electrodes are often too large or expensive for microreactor applications.
• Real-time pH monitoring is crucial for optimizing fermentation processes.
• Chemiresistive sensors provide a compact solution for pH measurement.
• This technology can facilitate advancements in biogas production and biomask conversion.

Purpose of Study

• To develop a small-scale pH sensor suitable for microreactor environments.
• To enable real-time monitoring of pH during fermentation processes.
• To reduce costs and size constraints associated with traditional pH measurement methods.

Methods Used

• Fabrication of Nafion-coated, polyaniline-functionalized sensors.
• Electrochemical reduction of graphene oxide.
• Testing sensor performance during Lactococcus lactis fermentation.
• Comparison with conventional pH measurement techniques.

Main Results

• The developed sensor effectively detects pH changes in real-time.
• It demonstrates advantages in size and cost over traditional pH electrodes.
• The sensor's performance is suitable for multiple microreactor applications.
• Insights gained can enhance bioprocess optimization.

Conclusions

• This chemiresistive micro pH sensor is a promising tool for bioprocess technology.
• It can significantly improve the efficiency of fermentation monitoring.
• The method has potential applications in various chemical and biological processes.

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