Probiotic Studies in Neonatal Mice Using Gavage

作者： Freddy Francis1, Natallia Varankovich2, Byron Brook1, Nelly Amenyogbe1, Rym Ben-Othman2, Bing Cai2, Danny Harbeson1, Aaron C. Liu1, Ben Dai2, Shelly McErlane4, Kris Andrews4, Tobias R. Kollmann*1,2, Pinaki Panigrahi*3 1Department of Experimental Medicine,University of British Columbia, 2Department of Pediatrics,University of British Columbia, 3Department of Epidemiology and Pediatrics,University of Nebraska Medical Centre, 4Animal Care Services,University of British Columbia

简介：

Overview

This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include probiotic dosing, methods of administration, and quantification of bacteria in the intestines.

Key Study Components

Area of Science

• Neuroscience
• Microbiology
• Animal Research

Background

• Probiotics can influence gut microbiome development.
• Gavage is a common method for administering substances to small animals.
• Neonatal mice are often used in microbiome studies.
• Proper handling techniques are crucial to minimize stress on the animals.

Purpose of Study

• To establish a protocol for gavaging probiotics in neonatal mice.
• To ensure accurate dosing and minimize stress during the procedure.
• To collect intestinal samples for microbiome analysis.

Methods Used

• Intra-esophageal gavage technique for probiotic administration.
• Use of sterile tools and methods to prevent contamination.
• Monitoring health signs of neonatal mice during and after gavage.
• Collection of intestinal samples post-euthanasia for analysis.

Main Results

• Successful gavage resulted in visible dye in the stomach.
• Health monitoring indicated recovery signs post-gavage.
• Proper technique minimized stress and risk of abandonment.
• Intestinal samples were collected for further analysis.

Conclusions

• The protocol established is effective for probiotic administration in neonatal mice.
• Careful handling and monitoring are essential for animal welfare.
• Further studies can build on this method to explore gut microbiome effects.

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