Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

作者： Moe Ishihara*1, Junhui Hu*1, Xiaoyu Zhang2, YongHyeon Choi3, Anthony Wong4, Celine Cano-Ruiz5, Rongwei Zhao6, Ping Tan7, Jonathan L. Tso1, Lily Wu1,8 1Department of Molecular and Medical Pharmacology,David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Bioengineering,Hanyang University, 4Department of Ecology and Evolutionary Biology,University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 6School of Life Sciences,Beijing Normal University, 7Department of Urology, West China Hospital,Sichuan University, 8Department of Urology,David Geffen School of Medicine, University of California, Los Angeles

简介：

Overview

This article presents two novel animal models for metastatic clear cell renal cell carcinoma (CCRCC), addressing the lack of comprehensive models for preclinical investigation. The models include an orthotopically implanted mouse model and a chicken chorioallantoic membrane model, both demonstrating lung metastasis similar to clinical cases.

Key Study Components

Area of Science

• Oncology
• Animal Models
• Metastasis Research

Background

• Clear cell renal cell carcinoma (CCRCC) is the most common subtype of kidney cancer.
• Localized CCRCC has a favorable outcome with surgery.
• One third of CCRCC patients develop distant metastasis, particularly to the lung.
• There is currently no effective therapy for this advanced stage of the disease.

Purpose of Study

• To develop and validate new animal models for studying CCRCC metastasis.
• To facilitate preclinical investigations into the disease mechanisms.
• To improve understanding of lung metastasis in CCRCC.

Methods Used

• Orthotopic implantation of tumor cells in mice.
• Utilization of the chicken chorioallantoic membrane model.
• Observation of lung metastasis development in both models.
• Comparison of model outcomes with clinical cases.

Main Results

• Both animal models successfully demonstrated lung metastasis.
• The models closely resemble clinical cases of CCRCC.
• These models provide a platform for further research into therapeutic strategies.
• Insights into the molecular mechanisms of metastasis were gained.

Conclusions

• The newly developed models are valuable for preclinical studies of CCRCC.
• They enhance the understanding of metastatic processes in kidney cancer.
• Future research can leverage these models to explore potential therapies.

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