Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

作者： Chi-Hao Hsiao1,2,3, Ya-Hsu Chiu4, Shao-Chih Chiu4,5, Der-Yang Cho4,6,7, Liang-Ming Lee1,2, Yu-Ching Wen1,2, Jia-Jhe Li4, Ping-Hsiao Shih4 1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital

简介：

Overview

This article presents a protocol for isolating and expanding cytokine-induced killer T cells derived from peripheral blood mononuclear cells (PBMCs). It also demonstrates their cytotoxic effects against various cancer cells using flow cytometry.

Key Study Components

Area of Science

• Immunology
• Cancer Biology
• Cell Therapy

Background

• Cytokine-induced killer (CIK) cells are a promising therapeutic option for cancer treatment.
• Isolation and expansion of PBMC-derived CIK cells can enhance their cytotoxic potential.
• Flow cytometry is a valuable tool for assessing cell viability and cytotoxicity.
• Standardized protocols are essential for reproducibility in clinical and research settings.

Purpose of Study

• To optimize a method for the expansion of PBMC-derived CIK cells.
• To evaluate the cytotoxicity of these cells against hematological and solid tumors.
• To provide a standard operating procedure for technicians and clinicians.

Methods Used

• Preparation of CIK cells from PBMCs.
• Washing and centrifugation of CIK cells.
• Resuspension of cells in sterile PBS.
• Cell counting and viability testing using the trypan blue exclusion assay.

Main Results

• Successful isolation and expansion of CIK cells were achieved.
• CIK cells demonstrated significant cytotoxicity against cancer cells.
• The method provides a reliable approach for quantifying CIK cell potency.
• Flow cytometry effectively assessed the cytotoxic effects of CIK cells.

Conclusions

• The optimized protocol enhances the clinical applicability of CIK cells.
• This method can facilitate further research and clinical evaluations.
• Standardized procedures are crucial for consistent results in cell therapy.

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