简介：
Overview
This article presents a protocol for isolating and culturing neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre +/- ;Rosa26 RFP/+ mice. The methodology allows for the study of adipogenesis and lipogenesis in vitro.
Key Study Components
Area of Science
- Neuroscience
- Stem Cell Biology
- Adipogenesis
Background
- NCADSCs are derived from neural crest cells.
- These cells provide an accessible source for studying adipose tissue.
- The protocol is designed for use with young mice.
- Experience with cautery and induced adipogenesis is critical for success.
Purpose of Study
- To isolate and culture NCADSCs for research purposes.
- To model adipogenesis and lipogenesis in vitro.
- To test drugs against obesity and cardiovascular diseases.
Methods Used
- Isolation of NCADSCs from periaortic adipose tissue.
- Fluorescence labeling of cells for FACS or magnetic sorting.
- Use of young mice for optimal cell yield.
- Demonstration of the procedure by lab personnel.
Main Results
- The protocol yields abundant NCADSCs for in vitro studies.
- Fluorescent labeling enhances cell sorting efficiency.
- Successful adipogenic induction observed in cultured cells.
- Method applicable for drug testing related to obesity.
Conclusions
- The protocol is effective for obtaining NCADSCs.
- It provides a valuable tool for studying adipogenesis.
- Potential applications in obesity and cardiovascular research.
What are NCADSCs?
NCADSCs are neural crest derived adipose-derived stem cells used for studying adipogenesis.
How are NCADSCs isolated?
They are isolated from the periaortic adipose tissue of specific mouse models.
What is the significance of using young mice?
Young mice provide a better yield of NCADSCs for research purposes.
What methods are used for cell sorting?
Fluorescence-activated cell sorting (FACS) and magnetic activated cell sorting are used.
What applications does this protocol have?
It can be used for modeling adipogenesis and testing drugs for obesity.
Who demonstrates the protocol?
The procedure is demonstrated by lab personnel, including a graduate student and a technician.
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