Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

作者： Nan Chen1,2, Kai Zhu1,2, Shiqiang Yan3,4, Junmin Li1,2, Tianyi Pan4, Mieradilijiang Abudupataer1,2, Fazle Alam4, Xiaoning Sun1,2, Li Wang3,4, Chunsheng Wang1,2 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital,Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College,Fudan University

简介：

Overview

This study presents a novel multilayered strategy for liquid-like bioinks, specifically gelatin methacryloyl with low viscosity, aimed at preventing the sedimentation of encapsulated cells. The approach enhances cell dispersity and maintains a suitable environment for cell viability during 3D printing.

Key Study Components

Area of Science

• Bioengineering
• Tissue Engineering
• 3D Bioprinting

Background

• Liquid-like bioinks are crucial for 3D printing in tissue engineering.
• Cell sedimentation can negatively impact the quality of printed constructs.
• Maintaining a suitable environment for cells during printing is essential.
• Interfacial tension can be utilized to prevent sedimentation.

Purpose of Study

• To develop a bioink that prevents cell sedimentation during 3D printing.
• To ensure a homogeneous distribution of cells within the bioink.
• To create functional in vitro tissue molds for drug screening and regenerative medicine.

Methods Used

• Preparation of gelatin methacryloyl bioink at a 10% concentration.
• Use of methacrylic anhydride in a specific weight ratio with gelatin.
• Sterilization of materials using 0.22-micrometer syringe filters.
• Control of temperature during the preparation process.

Main Results

• The multilayering technique promotes homogeneous cell dispersity.
• Cell-suitable bioink environment is maintained during printing.
• 3D constructs exhibit a uniform distribution of encapsulated cells.
• This method provides a viable option for in vitro tissue modeling.

Conclusions

• The developed bioink strategy effectively prevents cell sedimentation.
• It enhances the quality of 3D printed tissue constructs.
• This approach has significant implications for regenerative medicine.

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