An Orthotopic Resectional Mouse Model of Pancreatic Cancer

作者： Tony C. Y. Pang1,2,4,5, Zhihong Xu1,2, Alpha Raj Mekapogu1,2, Srinivasa Pothula1,2, Therese M. Becker3, David Goldstein1, Romano C. Pirola1,2, Jeremy S. Wilson1,2, Minoti V. Apte1,2 1Pancreatic Research Group, South Western Sydney Clinical School,University of New South Wales, 2Ingham Institute for Applied Medical Research, 3Centre for Circulating Tumour Cell Diagnostics and Research,Ingham Institute for Applied Medical Research, 4Surgical Innovations Unit,Westmead Hospital, 5Westmead Clinical School,University of Sydney

简介：

Overview

This protocol describes a robust and reproducible surgical resection model of pancreatic cancer in mice, which may be used for testing adjuvant and neoadjuvant therapy. The technique combines a co-infection orthotopic model with a safe pancreatic resection method.

Key Study Components

Area of Science

• Oncology
• Animal Models
• Surgical Techniques

Background

• Localized pancreatic cancer often requires surgical intervention.
• Adjuvant therapies are critical for improving patient outcomes.
• Existing models may not adequately replicate human disease.
• This study aims to enhance the modeling of pancreatic cancer in preclinical settings.

Purpose of Study

• To establish a safe and effective method for modeling localized pancreatic cancer.
• To facilitate the testing of therapeutic strategies in a controlled environment.
• To provide a reproducible surgical model for future research.

Methods Used

• Orthotopic implantation of pancreatic cancer in nude mice.
• Distal pancreatectomy and splenectomy procedures.
• Use of KPC tumor cells in an alternative immunocompetent model.
• Standardized surgical techniques for consistency.

Main Results

• The model effectively recapitulates human pancreatic cancer.
• Demonstrated safety and reproducibility of the surgical technique.
• Potential for testing both adjuvant and neoadjuvant therapies.
• Alternative models may provide insights into immune therapies.

Conclusions

• This protocol offers a valuable tool for pancreatic cancer research.
• It supports the development of new therapeutic strategies.
• Future studies can build on this model to enhance understanding of pancreatic cancer.

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