Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

作者： Noemie Bricmont1, Lionel Benchimol2, Anne-Lise Poirrier2, Christine Grignet3, Claire Seaton4, Mark A. Chilvers4, Marie-Christine Seghaye5, Renaud Louis1,6, Philippe Lefebvre2, Celine Kempeneers1,7 1Pneumology laboratory, I3 Group, GIGA Research Center,University of Liège, 2Department of ENT,University Hospital of Liège, 3Department for Occupational Safety and Health, Biosafety and Biosecurity Unit,University of Liège, 4Division of Respirology, Department of Pediatrics,University of British Columbia and British Columbia Children's Hospital, 5Division of Cardiology, Department of Pediatrics,University Hospital of Liège, 6Department of Pneumology,University Hospital of Liège, 7Division of Respirology, Department of Pediatrics,University Hospital of Liège

简介：

Overview

This article discusses the importance of precise respiratory epithelium sampling and processing for high-quality ciliary functional analysis in diagnosing primary ciliary dyskinesia (PCD). It highlights the adaptations made to the ciliary videomicroscopy protocol during the COVID-19 pandemic to ensure continued diagnostic services.

Key Study Components

Area of Science

• Neuroscience
• Respiratory Medicine
• Diagnostic Techniques

Background

• Digital high-speed videomicroscopy is a sensitive and specific method for diagnosing PCD.
• Current confirmatory tests for PCD include electron microscopy and genetic testing.
• These confirmatory tests may miss PCD diagnosis in 15 to 30% of patients.
• Standardization in operating procedures is lacking for digital high-speed videomicroscopy.

Purpose of Study

• To improve diagnostic accuracy for primary ciliary dyskinesia.
• To adapt protocols to ensure safety during the COVID-19 pandemic.
• To promote the use of digital high-speed videomicroscopy as a diagnostic tool.

Methods Used

• High-speed videomicroscopy for ciliary functional evaluation.
• Implementation of infection control measures in the protocol.
• Comparison of digital high-speed videomicroscopy with other diagnostic tests.
• Assessment of sensitivity and specificity in diagnosing PCD.

Main Results

• Digital high-speed videomicroscopy shows higher sensitivity and specificity for PCD diagnosis.
• Results can be obtained within a day, making it a practical diagnostic option.
• Adaptations made during the pandemic allow for continued diagnostic services.
• Standardization is necessary for broader acceptance of this method as a confirmatory test.

Conclusions

• Digital high-speed videomicroscopy is a valuable tool for diagnosing primary ciliary dyskinesia.
• Infection control measures are crucial for safe diagnostic practices during the pandemic.
• Further standardization of procedures is needed to enhance diagnostic reliability.

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