Creation and Maintenance of a Living Biobank – How We Do It

作者： Florian Bürtin1, Stephanie Matschos2, Friedrich Prall3, Christina S. Mullins2, Mathias Krohn2, Michael Linnebacher2 1Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, University of Rostock, 2Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, 3Institute of Pathology,University Medical Center Rostock

简介：

Overview

This article outlines the procedures for establishing a biobank specifically for colorectal and pancreatic cancer. It emphasizes the importance of integrating patient-derived models for functional testing in precision medicine.

Key Study Components

Area of Science

• Biobanking
• Cancer Research
• Precision Medicine

Background

• Traditional biobanks contain non-viable samples.
• Patient-derived models enhance preclinical assays.
• Functional testing is crucial for therapeutic strategies.
• Sample acquisition requires informed consent and adequate tumor size.

Purpose of Study

• To establish a living biobank for colorectal and pancreatic cancer.
• To enable functional testing for precision medicine.
• To improve the quality of samples for research.

Methods Used

• Sample acquisition and processing of blood and tissue.
• Isolation of mononuclear cells and tissue cubes for storage.
• Cell culture techniques for tumor outgrowth.
• Generation of patient-derived xenografts in mice.

Main Results

• Successful establishment of a biobank with viable samples.
• Integration of patient-derived models for enhanced testing.
• Effective methods for tissue processing and storage.
• Demonstrated feasibility of generating xenografts.

Conclusions

• The biobank provides a valuable resource for cancer research.
• Patient-derived models are essential for precision medicine.
• Methodologies established can be adapted for other cancers.

Frequently Asked Questions