The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

作者： Bram Meijlink1, Ilya Skachkov1, Antonius F. W. van der Steen1,2, Nico de Jong1,2, Klazina Kooiman1 1Department of Biomedical Engineering, Thoraxcenter,Erasmus MC University Medical Center Rotterdam, 2Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences,Delft University of Technology

简介：

Overview

This protocol describes the preparation and use of 5 to 8-day old chicken embryos and their chorioallantoic membrane (CAM) as an in vivo model for studying contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Ultrasound Imaging

Background

• Chicken embryos provide a viable in vivo model for research.
• The chorioallantoic membrane is accessible for experimental manipulation.
• This model allows for the study of drug delivery and imaging techniques.
• Basic lab tools are sufficient for embryo preparation.

Purpose of Study

• To investigate ultrasound imaging techniques.
• To explore microbubble-mediated drug delivery methods.
• To assess the viability and functionality of the chorioallantoic membrane.

Methods Used

• Preparation of chicken embryos from fertilized eggs.
• Techniques for accessing the chorioallantoic membrane.
• Use of syringes for albumin withdrawal and PBS replacement.
• Microscopy imaging of the embryo and CAM samples.

Main Results

• Successful extraction of embryos and CAM for imaging studies.
• Demonstrated viability of embryos post-extraction.
• Effective methods for drug delivery using microbubbles.
• Insights into blood flow imaging and ultrasound contrast agents.

Conclusions

• The chicken embryo model is effective for ultrasound research.
• Microbubble-mediated techniques show promise for drug delivery.
• This protocol can be adapted for various experimental needs.

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