Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

作者： Catherine M. Meis*1, Abigail K. Grosskopf*2, Santiago Correa1, Eric A. Appel1,3,4 1Department of Materials Science & Engineering,Stanford University, 2Department of Chemical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Department of Pediatrics - Endocrinology,Stanford University

简介：

Overview

This protocol describes the formulation of injectable polymer-nanoparticle (PNP) hydrogels for various biomedical applications. These hydrogels enable non-invasive drug and cell delivery, with potential uses in cancer treatment and tissue regeneration.

Key Study Components

Area of Science

• Biomaterials
• Drug Delivery
• Cell Encapsulation

Background

• PNP hydrogels can be injected through small needles.
• They self-heal quickly after injection.
• These materials can stabilize biopharmaceuticals.
• They have applications in localized therapy and extended drug release.

Purpose of Study

• To synthesize and characterize PNP hydrogels.
• To explore their potential in drug delivery and cell therapy.
• To assess their mechanical and thermal properties.

Methods Used

• Preparation of nanoparticles via nano precipitation.
• Formulation of hydrogels using specific polymer and nanoparticle concentrations.
• Rheological testing to measure mechanical properties.
• Drug release studies using capillary tubes.

Main Results

• Hydrogels exhibited shear-thinning and self-healing properties.
• Characterization revealed solid-like behavior in stiffer formulations.
• Encapsulated cells maintained viability and functionality.
• PNP hydrogels effectively stabilized sensitive cargo.

Conclusions

• PNP hydrogels are promising for drug delivery and cell therapy.
• They can enhance the stability and release profiles of therapeutic agents.
• Further research is needed to optimize formulations for clinical applications.

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