3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

作者： Tiankun Liu*1,2, Yongchun Shao*1,2, Zitong Wang1,2, Yuqiu Chen1,2, Yuan Pang1,2, Ding Weng1,2, Wei Sun1,2,3 1Biomanufacturing Center, Dept. of Mechanical Engineering,Tsinghua University, 2Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, 3Department of Mechanical Engineering,Drexel University

简介：

Overview

This article presents a mild bioprinting technique for creating hydrogel microcarriers with excellent controllability and biocompatibility. The process allows for the encapsulation of cells within microcarriers, which can be utilized for in vitro cell expansion and functional microtissue construction.

Key Study Components

Area of Science

• Bioprinting
• Cell culture
• Tissue engineering

Background

• Microcarriers are essential for cell culture and tissue engineering applications.
• Conventional methods may compromise the physical and chemical properties of bio-inks.
• There is a need for improved techniques that maintain cell viability and functionality.
• This study explores a novel approach using mild printing forces.

Purpose of Study

• To develop a bioprinting method for producing microcarriers.
• To ensure high biocompatibility and controllability during the printing process.
• To facilitate the encapsulation of cells for subsequent applications.

Methods Used

• Utilization of homemade nozzles for flexibility in material and diameter.
• Printing process driven by alternating viscous-inertial forces.
• Preparation of nozzles using glass micropipettes and microforging techniques.
• Sterilization of nozzles to ensure a clean working environment.

Main Results

• Microcarriers with diameters ranging from 50-500 µm were successfully produced.
• The printing process maintained the original properties of the bio-ink.
• Internal cell distribution and encapsulated structures were achieved.
• The method supports the assembly of various tissue structures.

Conclusions

• The mild bioprinting technique is effective for creating functional microcarriers.
• It offers a promising approach for in vitro applications in cell expansion and tissue engineering.
• Future studies may explore the application of these microcarriers in complex tissue models.

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