Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Overview

This study presents a protocol for implanting a chronic cranial window for longitudinal imaging of brain cells in awake, head-restrained mice. The method allows for repeated visualization of neuronal and astrocytic structure and activity over multiple sessions, facilitating the investigation of alterations in neurodevelopmental or neurodegenerative disorder models.

Key Study Components

Area of Science

• Neuroscience
• Imaging Techniques
• Neurobiology

Background

• The study focuses on imaging brain cells to monitor their structure and function longitudinally.
• It utilizes a chronic cranial window to facilitate repeated sessions of imaging in live mice.
• This approach aids in understanding neuronal changes in various neurological conditions.

Purpose of Study

• To enable the chronic imaging of neuronal and astrocytic interactions.
• To assess structural and functional changes in models of neurological disorders.
• To understand how learning affects neuronal and astrocytic dynamics.

Methods Used

• The main platform used is in vivo imaging of the brain in head-restrained mice.
• The biological model involves head-restrained mice that undergo cranial window implantation.
• No multiomics workflows are mentioned.
• Critical steps include careful bone thinning and precise placement of the cover glass during the procedure.
• Surgical techniques include viral injections and secure closure using adhesives and dental cement.

Main Results

• The quality of cranial windows was assessed by imaging dendritic structures and astrocytic calcium activity over time.
• Initial findings demonstrated new dendritic spines appearing during the imaging sessions.
• Temporal dynamics of calcium activity in astrocytes were captured during behavioral tasks.
• Two critical procedural steps highlighted the importance of bone thinning and glass placement for successful imaging.

Conclusions

• This study demonstrates a reliable protocol for chronic imaging in live mice, aiding the understanding of brain plasticity.
• The method facilitates longitudinal studies of cellular dynamics, crucial for exploring neuronal mechanisms in diseases.
• The implications extend to understanding how brain activity and structure are affected by learning experiences.

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