Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

作者： Keir A. Forgie1,2, Nicholas Fialka3, Mubashir Khan4, Max Buchko1,2, Sanaz Hatami4, Sayed Himmat4, Xiao Qi4, Xiuhua Wang4, Katie-Marie Buswell5, Ryan Edgar5, Daina Domahidi5, Darren H. Freed1,2,6,7, Jayan Nagendran1,2,6,7 1Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine,University of Alberta, 2Mazankowski Alberta Heart Institute, 3Faculty of Medicine and Dentistry,University of Alberta, 4Department of Surgery, Faculty of Medicine,University of Alberta, 5Ray Rajotte Surgical-Medical Research Institute (SMRI),University of Alberta, 6Alberta Transplant Institute, 7Canadian Donation and Transplantation Research Program

简介：

Overview

This protocol outlines a juvenile porcine model for orthotopic left lung allotransplantation, emphasizing anesthetic and surgical techniques. It serves as a valuable method for ESLP research and includes critical steps and troubleshooting tips.

Key Study Components

Area of Science

• Neuroscience
• Transplantation Biology
• Large Animal Models

Background

• This protocol is designed for large animal models, specifically juvenile pigs.
• It provides detailed surgical management for lung transplantation.
• The technique is beneficial for ex-situ lung perfusion research.
• It allows for validation of ex-situ findings with an in vivo model.

Purpose of Study

• To describe a straightforward lung transplant protocol.
• To assess isolated left lung function post-transplant.
• To stabilize the pig during surgery.

Methods Used

• Positioning the pig in a right lateral decubitus position.
• Performing a left anterior lateral thoracotomy.
• Identifying anatomical landmarks for incision.
• Conducting the transplant procedure with emphasis on surgical techniques.

Main Results

• The protocol provides a clear surgical approach for lung transplantation.
• It facilitates assessment of lung function in a controlled environment.
• Stabilization techniques are effective during the procedure.
• The method supports future research in artificial organ testing.

Conclusions

• This juvenile porcine model is a valuable tool for lung transplantation research.
• It enhances understanding of lung function and surgical management.
• The protocol can be adapted for various research applications.

Frequently Asked Questions