Establishment of a Mouse Severe Acute Pancreatitis Model using Retrograde Injection of Sodium Taurocholate into the Biliopancreatic Duct

作者： Xiaochong Zhou1, Haifeng Chen1, Xing Wei1, Yang He2,4,5, Chunfang Xu1, Zhen Weng2,3,4,6 1Department of Gastroenterology,the First Affiliated Hospital of Soochow University, 2MOE Engineering Center of Hematological Disease,Soochow University, 3Cyrus Tang Hematology Center,Soochow University, 4National Clinical Research Center for Hematologic Diseases,the First Affiliated Hospital of Soochow University, 5MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology,The First Affiliated Hospital of Soochow University, 6Collaborative Innovation Center of Hematology,Soochow University

简介：

Overview

This article describes a rapid and accessible mouse model for studying severe acute pancreatitis through retrograde injection of sodium taurocholate. The model allows for the investigation of molecular mechanisms and therapeutic interventions in acute pancreatitis.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Pathophysiology

Background

• Acute pancreatitis is a serious condition that requires effective models for study.
• Existing models may be complex and time-consuming.
• This study aims to simplify the process of inducing acute pancreatitis in mice.
• Understanding the disease mechanisms can lead to better therapeutic strategies.

Purpose of Study

• To establish a reliable mouse model of severe acute pancreatitis.
• To facilitate the study of disease mechanisms and treatment options.
• To provide a cost-effective method using readily available reagents.

Methods Used

• Retrograde injection of sodium taurocholate into the biliopancreatic duct.
• Animal procedures approved by the Animal Ethics Committee.
• Use of anesthesia and surgical techniques to induce pancreatitis.
• Histological evaluation of pancreatic tissues post-operation.

Main Results

• Severe acute pancreatitis was successfully induced in mice.
• Histological analysis showed necrosis in pancreatic tissues.
• Significant differences in serum enzyme levels between control and SAP mice.
• The model demonstrated high survival rates and reproducibility.

Conclusions

• The described model is effective for studying severe acute pancreatitis.
• It allows for rapid training of novice surgeons in the procedure.
• This approach can enhance research into therapeutic interventions.

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