Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

作者： Youjin Lee*1,2, Daeho Ryu*3, Saeyeong Jeon3, Yena Lee1, Yoon Kyung Cho1, Chang-Hyeon Ji1,2, Yong-Kweon Kim3,4, Sang Beom Jun1,2,5 1Department of Electronic and Electrical Engineering,Ewha Womans University, 2Graduate Program in Smart Factory,Ewha Womans University, 3Department of Electrical and Computer Engineering,Seoul National University, 4Graduate School of Engineering Practice,Seoul National University, 5Department of Brain and Cognitive Sciences,Ewha Womans University

简介：

Overview

This study presents a novel optrode system that integrates optical fibers for light delivery and an electrode array for neural recording. The feasibility of this system is demonstrated through in vivo experiments conducted on transgenic mice expressing channelrhodopsin-2, enabling simultaneous optogenetic stimulation and electrophysiological recording.

Key Study Components

Area of Science

• Neuroscience
• Electrophysiology
• Optogenetics

Background

• Optogenetics is a technique for understanding neurological diseases.
• The study aims to enhance neural recording capabilities.
• Wireless systems are crucial for broader applications in optogenetics.
• LEDs provide advantages over lasers in terms of simplicity and cost.

Purpose of Study

• To fabricate an efficient optrode system for light stimulation and recording.
• To investigate neural responses related to opting light stimulation.
• To demonstrate the applicability of the system in freely moving animals.

Methods Used

• An LED-based optrode system was developed for simultaneous functioning.
• Transgenic mice expressing channelrhodopsin-2 served as the biological model.
• In vivo experiments involved surgical procedures to position the device.
• Measurement of light intensity and various surgical manipulations were carried out.
• Data acquisition, including neural spike sorting and analysis, was performed using MATLAB.

Main Results

• The study reveals enhanced evoked neural spikes during light stimulation compared to baseline.
• Significant increases in individual neural spikes following light pulses were recorded.
• Does show the potential for this system to study neuro signaling linked to behavioral responses.
• The system minimizes artifact noise due to its design and configuration.

Conclusions

• This study demonstrates that the LED-based optrode system effectively combines optogenetic stimulation and neural recording.
• The findings show potential for application in researching various neurobiological phenomena.
• Implications include investigating pathology and treatment mechanisms of neurological conditions.

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