A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

作者： Victor Pozzo1,2,4, Golda Romano1,2,4, Marion Goutard1,2,4, Elise Lupon1,2,4, Pierre Tawa1,2,4, Aylin Acun3,4,5, Alec R. Andrews2, Corentin B. Taveau1,2,4,6, Basak E. Uygun1,2,3,4, Mark A. Randolph1,2,4, Curtis L. Cetrulo1,2,4, Alexandre G. Lellouch1,2,4,6 1Division of Plastic and Reconstructive Surgery,Massachusetts General Hospital, Harvard Medical School, 2Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences,Massachusetts General Hospital, Harvard Medical School, 3Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, Harvard Medical School, 4Shriners Hospital for Children, 5Department of Biomedical Engineering,Widener University, 6Service de Chirurgie Plastique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris (APHP),Université Paris Descartes

简介：

Overview

This protocol outlines the porcine fascio-cutaneous flap model, emphasizing its reliability in skin vascularization. It serves as a valuable tool for studying vascularized composite tissue in various research fields.

Key Study Components

Area of Science

• Vascularized composite tissue research
• Surgical techniques in large animal models
• Tissue engineering

Background

• Fasciocutaneous flaps are essential for studying skin vascularization.
• Swine models provide a reliable platform for surgical research.
• The technique aids in understanding tissue engineering and immunology.
• Machine perfusion studies can be conducted using this model.

Purpose of Study

• To describe the surgical technique for creating fasciocutaneous flaps in swine.
• To demonstrate the model's utility in vascularized composite tissue research.
• To provide a reliable method for studying skin vascularization.

Methods Used

• Palpation of the saphenous artery for flap design.
• Identification of flap limits based on anatomical landmarks.
• Execution of the surgical technique for flap creation.
• Application of the model in various research contexts.

Main Results

• The technique ensures reliable skin vascularization.
• Facilitates studies in tissue engineering and immunology.
• Provides a robust model for machine perfusion research.
• Enhances understanding of vascularized composite tissue dynamics.

Conclusions

• The porcine fascio-cutaneous flap model is a valuable research tool.
• It offers insights into vascularized composite tissue applications.
• The model supports advancements in surgical techniques and tissue engineering.

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