Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

作者： Thanh Nguyen1, Sang Joon Ahn2, Richard West3, Ibra S. Fancher1 1Department of Kinesiology and Applied Physiology, College of Health Sciences,University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine,University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute,University of Delaware

简介：

Overview

This protocol describes a method for dissecting mouse adipose depots and isolating arterial endothelial cells, providing insights into vascular cell biology and dysfunction mechanisms.

Key Study Components

Research Area

• Vascular cell biology
• Mechanisms of vascular dysfunction
• Animal models of disease

Background

• Understanding endothelial cell populations is crucial for vascular research.
• The method can be adapted for various vascular beds.
• Optimizing the digestion protocol is critical for accurate results.

Methods Used

• Dissection and isolation of adipose tissue and arteries
• Mouse model used for in vivo studies
• Flow cytometry for cell identification

Main Results

• Identified endothelial cells using specific markers (CD31 positive, CD45 negative).
• Understood contributions of different vascular beds to cell populations.
• Validated isolation and digestion protocols for functional tests.

Conclusions

• The study provides a valuable method for investigating vascular biology.
• Enhances understanding of vascular dysfunction in disease contexts.

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