Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

作者： Kiara L. Lacy1, Samuel Salib1, Mary Tran2, Tunglin Tsai1, Rominna Valentine1, Herdeline Ann M. Ardoña1,2,3,4, Tayloria N. G. Adams1,2,4 1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering,University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering,University of California, Irvine, 3Department of Chemistry, School of Physical Sciences,University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center,University of California, Irvine

简介：

Overview

This article presents a light-induced dielectrophoresis (LiDEP) protocol for the characterization of human mesenchymal stem cells (hMSCs). The method allows for real-time adjustments to the virtual electrode configuration, enhancing the analysis of stem cell heterogeneity without altering the cells' native state.

Key Study Components

Area of Science

• Cell biology
• Microfluidics
• Electrophysiology

Background

• Human mesenchymal stem cells (hMSCs) exhibit significant heterogeneity.
• Traditional methods for characterizing cell behavior can alter the native state of cells.
• LiDEP provides a label-free approach to study these cells.
• The technique utilizes a microfluidic device with a photoconductive layer.

Purpose of Study

• To develop a protocol for characterizing hMSCs using LiDEP.
• To optimize the microfluidic device for real-time analysis.
• To enhance understanding of stem cell heterogeneity.

Methods Used

• Preparation of a microfluidic device with specific dimensions.
• Fabrication of the device using ITO glass and double-sided tape.
• Preparation of a DEP buffer solution with BSA.
• Application of AC electric fields to observe cell behavior under LiDEP.

Main Results

• Successful characterization of hMSCs without altering their state.
• Real-time adjustments to the virtual electrode configuration were achieved.
• Demonstrated the effectiveness of LiDEP in analyzing stem cell heterogeneity.
• Provided a detailed protocol for future researchers to replicate.

Conclusions

• LiDEP is a promising technique for studying heterogeneous cell populations.
• The protocol can be adapted for various cell types beyond hMSCs.
• This method may lead to advancements in stem cell research and applications.

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