Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model

作者： Sijuan Sun*1, Hongbin Zhu*2, Shoubao Wang3, Xiuxia Xu4, Lincai Ye2,5,6 1Department of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University, 2Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine,Shanghai Jiaotong University, 3Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine,Shanghai Jiao Tong University, 4Department of Radiology, Huangpu Branch, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine, 5Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University, 6Shanghai Institute for Pediatric Congenital Heart Disease, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University

简介：

Overview

This protocol presents the establishment and confirmation of a postnatal right ventricular volume overload (VO) model in mice with abdominal arteriovenous fistula (AVF), which can be applied to investigate how VO contributes to postnatal heart development.

Key Study Components

Area of Science

• Congenital heart disease
• Heart regeneration
• Postnatal heart development

Background

• Understanding the effects of volume or pressure overload on heart and lung development.
• Development of various postnatal mouse or rat models of congenital heart disease.
• Use of the AVF model to induce chronic heart VO in adult animals.
• Limited knowledge about the AVF model in postnatal mice.

Purpose of Study

• To investigate how volume overload affects postnatal heart development.
• To advance understanding of pediatric congenital heart disease.
• To establish a reliable model for future research.

Methods Used

• Establishment of a postnatal right ventricular volume overload model.
• Confirmation of the model's effectiveness.
• Application of the model to study heart development.
• Utilization of abdominal arteriovenous fistula (AVF) in mice.

Main Results

• Successful establishment of the volume overload model in postnatal mice.
• Insights into the impact of VO on heart development.
• Potential for further research into pediatric heart conditions.
• Contribution to the understanding of congenital heart disease mechanisms.

Conclusions

• The AVF model is a valuable tool for studying postnatal heart development.
• Volume overload has significant implications for heart health in pediatric populations.
• Further research is needed to explore the long-term effects of VO.

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