Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

作者： Xinjia Cai1,2, Heyu Zhang3, Jianyun Zhang1,2, Tiejun Li1,2 1Department of Oral Pathology,Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 2Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions,Chinese Academy of Medical Sciences (2019RU034), 3Central Laboratory,Peking University School and Hospital of Stomatology

简介：

Overview

This study focuses on the mechanisms of malignant transformation in oral potentially malignant disorders, particularly oral submucous fibrosis. It utilizes laser capture microdissection to extract cells from specific histological regions for multi-omics analysis.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Pathology

Background

• Oral potentially malignant disorders can progress to oral cancer.
• Current analyses often rely on frozen samples, while many tissues are formalin-fixed paraffin-embedded.
• There is a need for techniques that utilize paraffin samples for better resource utilization.
• Paraffin tissue samples are abundant and valuable for research.

Purpose of Study

• To develop experimental techniques for analyzing paraffin-embedded tissues.
• To understand disease mechanisms and malignant transformation.
• To leverage available resources for comprehensive studies.

Methods Used

• Laser capture microdissection of oral submucous fibrosis tissues.
• Multi-omics data analysis including genomic, transcriptomic, and proteomic approaches.
• Use of deep learning models for predictive analysis.
• Sectioning of tissues using a microtome.

Main Results

• Successful extraction of cells from histological regions of interest.
• Enhanced understanding of the progression from benign to malignant states.
• Development of techniques applicable to formalin-fixed samples.
• Potential for improved predictive models of oral cancer.

Conclusions

• The study provides a protocol for utilizing paraffin-embedded tissues.
• It highlights the importance of multi-omics analysis in understanding oral disorders.
• Future research can build on these findings to further explore malignant transformations.

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