A Mouse Abdominal Aortic Aneurysm Model by Periadventitial Calcium Chloride and Elastase Infiltration

作者： Jinting Ge*1, Chengxin Weng*1, Xiaoling Zhang2, Jichun Zhao1, Hua Zhang2, Ding Yuan1, Tiehao Wang1 1Division of Vascular Surgery, Department of General Surgery, West China Hospital,Sichuan University, 2Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital,Sichuan University

简介：

Overview

This study presents a protocol for establishing a mouse abdominal aortic aneurysm model using calcium chloride and elastase. This model is designed to investigate the pathophysiological mechanisms underlying abdominal aortic aneurysms.

Key Study Components

Area of Science

• Neuroscience
• Vascular Biology
• Pathophysiology

Background

• Abdominal aortic aneurysms (AAA) are significant vascular conditions.
• Existing models have limitations that this study aims to address.
• The proposed model combines advantages of previous methods.
• It demonstrates high incidence rates and pathophysiological changes similar to human AAA.

Purpose of Study

• To establish a reliable mouse model for AAA research.
• To facilitate the investigation of AAA pathogenesis.
• To develop therapeutic approaches for AAA and related diseases.

Methods Used

• Mouse anesthesia and positioning on a heating pad.
• Longitudinal incision along the midline of the abdomen.
• Accessing the peritoneal cavity via a transabdominal approach.
• Application of calcium chloride and elastase for aneurysm induction.

Main Results

• The model shows a high incidence rate of AAA.
• Pathophysiological changes resemble those observed in humans.
• The protocol is safe, stable, and reproducible.
• It allows for detailed investigation of AAA mechanisms.

Conclusions

• This model is a valuable tool for AAA research.
• It enhances understanding of AAA pathophysiology.
• Future studies can focus on therapeutic developments.

Frequently Asked Questions