In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker Disease

作者： Yutian Suo*1,2, Yinan Zhang*1,2, Zheng Li1,2, Mingzhuo Zhao3, Wanna Shen1,2, Yuchen Fu1,2, Donglin Wang1,2, Long Pan1,2, Xiaohua Su4, Jiaping Zhao1,2 1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration,Chinese Academy of Forestry, 2Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, 3Beijing Haidian Kaiwen Academy High School, 4State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry,Chinese Academy of Forestry

简介：

Overview

This study provides a protocol for assessing the resistance of poplar hybrids to stem canker pathogens using an in vivo leaf inoculation method. The method is particularly effective for large-scale screening of resistance to Cytospora chrysosperma and Botryosphaeria dothidea canker diseases in poplar breeding efforts in China.

Key Study Components

Research Area

• Pathogen resistance assessment
• Poplar hybrid breeding
• In vivo inoculation techniques

Background

• Importance of identifying disease-resistant strains
• The challenges of traditional methods for assessing disease resistance
• The need for efficient screening techniques in large-scale breeding programs

Methods Used

• In vivo leaf inoculation method
• Poplar as a biological model
• Cultivation of fungal pathogens and measurement of necrotic lesions

Main Results

• Significantly shortened disease resistance selection period to 2 years
• High efficiency in screening large-scale hybrid clones
• Provided detailed methodology for assessing pathogen impact and resistance

Conclusions

• This study demonstrates a novel approach to rapidly assess the resistance of poplar hybrids to stem cankers.
• The method has significant implications for accelerating poplar breeding programs aimed at improving disease resistance.

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