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BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells

作者： Adeline Cœugnet1, Hannes Schihada2, Estelle Rascol1, Isabel Alves1, Marie-Lise Jobin1 1CNRS, Bordeaux INP, CBMN, UMR 5248,University of Bordeaux, 2Department of Pharmaceutical Chemistry,Philipps-University Marburg
简介：

This protocol describes the use of BRET-based biosensors for real-time measurement of G protein activation in living HEK293 cells upon G protein-coupled receptor ligand stimulation. Here, the β2-adrenergic receptor and cannabinoid type 1 receptor serve as examples to demonstrate the efficiency of sensors for several G protein subtypes and across various GPCRs.

标签: G protein-coupled receptors,    GPCR activation,    heterotrimeric G proteins,    GDP-GTP exchange,    G protein dissociation,    bioluminescence resonance energy transfer,    BRET biosensors,    G-CASE biosensors,    single plasmid transfection,    HEK293T cells,   
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