We have developed a minimally invasive technique to create a rabbit ischemic ear wound model by dividing the central artery and nerve and the cranial neurovascular bundle. A subcutaneous tunnel then cuts all subcutaneous tissues. This procedure causes minimal skin disruption and can be safely used in diabetic animals.
The overall goal of the following experiment is to make it possible to create ischemic wounds, not only in normal rabbits, but also in rabbits with reduced resistance such as those which are aged or diabetic. All the currently used techniques for ischemic wound creation are highly invasive, and so far, none of these techniques can be used in diabetic animals. This procedure uses minimally invasive surgery that begins with making three small skin incisions over the three vascular bundles.
The next step is to isolate and protect the central vein and the coddle vascular bundle so that blood circulation to the ear is reduced but not totally eliminated. Finally, the remaining vessels, nerves, and subcutaneous tissues are all severed or ligated via the skin incisions. Ear ischemia is confirmed by the absence of arterial pulse, ear appearance, temperature difference, and subcutaneous oxygen saturation.
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