We describe a technique for labeling and tracking stem cells with FDA-approved, superparamagnetic iron oxide (SPIO), ferumoxytol (Feraheme). This cellular imaging technique that utilizes magnetic resonance (MR) imaging for visualization, is readily accessible for long-term monitoring and diagnosis of successful or unsuccessful stem cell engraftments in patients.
In this technique. In translational science cells are labeled using an FDA approved ultra-small super paramagnetic iron oxide called far amatol. Begin with human mesenchymal stem cells plated at 80%con fluency C, then transfect the contrast agent far amatol in serum free media to label the cells continue to harvest and enumerate the labeled H HMCs for experimental use.
Ultimately, magnetic resonance imaging results can show a significant negative contrast or T two effect from U-S-P-I-O labeled cells. Welcome to the DDR link lab at the Molecular Imaging Program at Stanford. The implications of this technique extends towards in vivo tracking of stem cell implants through direct visualization by non-invasive MR Imaging.
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