We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.
Cardiac derived cells are placed onto cell culture plates for simulated ischemia. Reperfusion model ischemia is achieved with 150 minutes in a glucose free medium, while the oxygen level is below 0.5%This ischemic period leads to the shrinking and blabbing of most cells. Then normal media and oxygen levels are reintroduced to simulate reperfusion.
Mesenchymal stem cells are added and cultivated together with the damaged cells. After 24 hours of co cultivation cells are stained with calcium and Ethereum homodimer To differentiate between live and dead cells, confocal microscope, it clearly shows that two cell populations and their interactions flow cytometry analysis reveals three clusters of damaged cells, which can be plotted on a graph and analyzed statistically Stem cell transplantation. Protocols are finding their way into clinical practice, getting better results, making the protocols more robust, and finding new sources for implantable cells are the focus of recent research.
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