Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
The overall goal of this procedure is to study mastitis or inflammation of the mammary gland, and more specifically to visualize and quantify NF Kary activity within an inflamed lactating gland. This is accomplished by first crossing a female NF Kappa b reporter transgenic with a wild type male. Eight days after the birth of the pups, lipopolysaccharide is introduced into the lactating gland of the female via an introductory injection technique, which does not damage all wound the site of injection six hours after injection.
The mouse undergoes bioluminescent imaging to detect luciferase activity within the mammary gland. Ultimately, results can show significant NF KB activity in the LPS treated glands through the use of the Intraductal injection method, followed by bioluminescent imaging. The main advantage of our approach over other methods of intraductal injection is that the site of injection is not damaged in any way.
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