Postoperative ileus (POI) is a complication of abdominal surgery leading to increased morbidity and a prolonged hospital stay. Because prophylactic or therapeutic strategies are lacking intensified research is necessary. Therefore we established a standardized and feasible mouse model to investigate the pathophysiology of POI and to study potential therapeutic options.
The overall goal of the following experiments is to perform an intestinal manipulation to study the development and dissolution of the postoperative ileus in a rodent model, this is achieved by standardized manipulation of the small bowel between two moist cotton applicators. Next, gastrointestinal and colonic transit are analyzed to measure postoperative motility. Then histochemical analysis and organ culture of the small bowel muscularis, externa, or me is performed to examine levels of inflammation.
Results are obtained that show a strong infiltration of neutrophil granulocytes into the me and excess production of nitric oxide in me cultures resulting in delays in gastrointestinal and colonic transit times. This method can help answer key questions concerning the development and the solution of postoperative ilios, and in investigating potential prophylaxis or therapy, Demonstrating the procedure will be Mario Lewison, a technician from our lab After administering 0.8 to 1%isoflurane in oxygen to a male mouse. Place the animal in a supine position and use adhesive tape to fix the extremities.
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