We demonstrate a protocol in which administration of the genotoxic agent azoxymethane (AOM) followed by three cycles of the pro-inflammatory agent dextran sulfate sodium (DSS) rapidly and consistently generates colon tumors in mice with morphologic and molecular similarities to those seen in human colitis-associated cancer.
On day zero, Myer injected with azoxymethane or a OM the following week. Their drinking water is replaced with a solution containing dextran sulfate sodium or DSS. At week three, mice are given water for the following two weeks.
This DSS water cycle is repeated two additional times. After the second DSS cycle. Mice can be assessed for tumors by mirroring endoscopy.
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