By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.
The overall goal of this procedure is to visualize initial stages of glioma initiation from single injected glioblastoma cells in vivo. This is accomplished by first thinning, an area of mouse skull down to a thickness suitable for in vivo imaging purpose. Next, the thin skull area is polished.
Then GFP labeled glioblastoma cells are injected into the mouse brain and the cranial window is sealed for in vivo imaging. Finally, the injected glioblastoma cells are imaged daily to visualize glioma initiation from injected glioblastoma cells in vivo. Ultimately, results can be obtained that show in vivo glioma initiation from a single injection of glioblastoma cells and its relationship with vascular endothelial cells through dual color in vivo two photon microscopy.
繁體中文
