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JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Journal
JoVE Encyclopedia of Experiments
神经科学
医学
免疫学与感染
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癌症
生物学
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10.3791/62328-v
11:32 min
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
10.3791/62245-v
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles (“Nanoblades”)
10.3791/62068-v
06:37 min
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
10.3791/57518-v
08:37 min
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
10.3791/63177-v
10:08 min
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
10.3791/61855-v
08:20 min
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
10.3791/59780-v
09:05 min
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
10.3791/59639-v
05:51 min
Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
10.3791/59382-v
10:10 min
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10.3791/59202-v
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
10.3791/58790-v
09:13 min
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
10.3791/56969-v
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
10.3791/53879-v
09:18 min
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
10.3791/52118-v
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
01:28 min
8.9: CRISPR/Cas9 Genome Editing
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