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Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells 10.3791/58715-v
Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays 10.3791/58710-v 11:05 min
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker 10.3791/58697-v 08:12 min
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos 10.3791/58598-v 07:24 min
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes 10.3791/58223-v
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
CRISPR Guide RNA Cloning for Mammalian Systems 10.3791/57998-v
CRISPR Guide RNA Cloning for Mammalian Systems
Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts 10.3791/57530-v 07:02 min
Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments 10.3791/56600-v 07:51 min
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism 10.3791/56189-v 11:37 min
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations 10.3791/55130-v 10:41 min
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides 10.3791/54863-v 09:58 min
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution 10.3791/54391-v 10:53 min
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly 10.3791/54064-v 09:51 min
Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications 10.3791/54063-v 08:23 min
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy 10.3791/53776-v
Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome 10.3791/53165-v 08:49 min
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
Electroretinogram Analysis of the Visual Response in Zebrafish Larvae 10.3791/52662-v 09:44 min
Electroretinogram Analysis of the Visual Response in Zebrafish Larvae
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development 10.3791/52496-v
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation 10.3791/52053-v 12:42 min
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants 10.3791/51718-v 09:16 min
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
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