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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution 10.3791/59202-v
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens 10.3791/59102-v 07:42 min
Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy 10.3791/58896-v 12:15 min
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
A Purification and In Vitro Activity Assay for a (p)ppGpp Synthetase from Clostridium difficile 10.3791/58547-v 09:53 min
A Purification and In Vitro Activity Assay for a (p)ppGpp Synthetase from Clostridium difficile
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity 10.3791/58100-v 07:46 min
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory 10.3791/58012-v
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes 10.3791/57141-v 06:58 min
Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases 10.3791/56975-v 08:57 min
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures 10.3791/55502-v
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
A Web Tool for Generating High Quality Machine-readable Biological Pathways 10.3791/54869-v
A Web Tool for Generating High Quality Machine-readable Biological Pathways
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay 10.3791/54443-v
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits 10.3791/54286-v C
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers 10.3791/54010-v 10:21 min
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways 10.3791/53850-v 09:27 min
Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes 10.3791/53168-v
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
2.1: Introduction to Catalysis 08:23 min
2.1: Introduction to Catalysis
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples 10.3791/52784-v 09:45 min
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
Assessing the Secretory Capacity of Pancreatic Acinar Cells 10.3791/51799-v 09:52 min
Assessing the Secretory Capacity of Pancreatic Acinar Cells
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water 10.3791/51414-v 12:50 min
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses 10.3791/51222-v 11:34 min
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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