Bacterial Gene Expression Analysis Using Microarrays

Hi, my name is Ena and We routinely do gene expression profiling in the lab. And we use this technique to monitor differential regulated genes between meth and wild type. And today I have a wild type and meth RNA samples and we all do C first.

We use a indirect labeling methods and the advantage of this technique is to prevent eye bias that can occur in direct labeling. And to do that we have RNA samples and we opt obtain three microgram of RNA in 13 microliter of to total volume. And we add 2.5 micro of random he in our parks.