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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE 10.3791/55448-v
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy 10.3791/51087-v
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
Miniaturized Sample Preparation for Transmission Electron Microscopy 10.3791/57310-v
Miniaturized Sample Preparation for Transmission Electron Microscopy
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy 10.3791/2909-v
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion 10.3791/63387-v 13:43 min
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography 10.3791/1846-v 09:23 min
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
Single Particle Cryo-Electron Microscopy: From Sample to Structure 10.3791/62415-v
Single Particle Cryo-Electron Microscopy: From Sample to Structure
Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation 10.3791/66197-v
Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope 10.3791/63519-v
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block 10.3791/62321-v
Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies 10.3791/65878-v 07:10 min
High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
Cryo-EM and Single-Particle Analysis with Scipion 10.3791/62261-v
Cryo-EM and Single-Particle Analysis with Scipion
Preparation of High-Temperature Sample Grids for Cryo-EM 10.3791/62772-v 05:05 min
Preparation of High-Temperature Sample Grids for Cryo-EM
LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation 10.3791/66831-v 06:12 min
LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation
Imaging ATG9A, a Multi-Spanning Membrane Protein 10.3791/65349-v
Imaging ATG9A, a Multi-Spanning Membrane Protein
Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon 10.3791/64136-v 04:52 min
Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples 10.3791/64099-v 07:27 min
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination 10.3791/64061-v 07:59 min
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Modeling Ligands into Maps Derived from Electron Cryomicroscopy 10.3791/66310-v 09:30 min
Modeling Ligands into Maps Derived from Electron Cryomicroscopy
ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography 10.3791/65828-v
ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography
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