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07:28 min
2.12: An Introduction to Transfection
10.3791/1499-v
11:42 min
Lentivirus Production
10.3791/59010-v
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
10.3791/51897-v
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
10.3791/59033-v
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
10.3791/675-v
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
10.3791/51960-v
Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
10.3791/51229-v
05:24 min
In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
10.3791/3024-v
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
10.3791/2444-v
Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
10.3791/51516-v
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
10.3791/3965-v
10:51 min
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
10.3791/3681-v
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
10.3791/3681-v
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
10.3791/2204-v
Determining Cell Number During Cell Culture using the Scepter Cell Counter
10.3791/58960-v
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
10.3791/3343-v
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3’UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
10.3791/3171-v
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
10.3791/2373-v
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
10.3791/2314-v
12:59 min
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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