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JoVE Encyclopedia of Experiments
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10.3791/65126-v
Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
10.3791/62409-v
09:40 min
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
10.3791/61839-v
09:31 min
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
10.3791/61786-v
14:51 min
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
10.3791/60703-v
07:08 min
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
10.3791/56688-v
08:55 min
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
10.3791/55585-v
10:23 min
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10.3791/55341-v
07:40 min
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
10.3791/54886-v
11:14 min
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
10.3791/51911-v
06:55 min
Protein Isolation from the Developing Embryonic Mouse Heart Valve Region
10.3791/51711-v
13:05 min
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
08:08 min
4.12: Co-Immunoprecipitation and Pull-Down Assays
07:29 min
2.5: Separating Protein with SDS-PAGE
01:15 min
32.13: Western Blotting
01:27 min
32.12: SDS-PAGE
10.3791/2455-v
Dissection of Human Vitreous Body Elements for Proteomic Analysis
10.3791/2164-v
Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
10.3791/1916-v
10:48 min
SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
10.3791/1431-v
10:57 min
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
10.3791/1350-v
07:47 min
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
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